highly significantP = 0.0184
Not only concentration and observation time but also the interaction between concentration and time had a highly significant influence on mean mortality (F = 3.34, df = 4, P = 0.0184; F = 12.51, df = 4, P < 0.001; F = 4.94, df = 9, p < 0.0002), respectively indicating that the effect of ivermectin concentration on tick mortality depended on observation time.