Gingiva-Derived Decellularised Extracellular Matrix Hydrogel Supports Osteogenic and Angiogenic Phenotypes of 3D STRO-1<sup>+</sup> GMSC/HUVEC Spheroids In Vitro.

However, when the concentration of G-dECM was 4 mg/mL, the mRNA and protein levels of VEGFA and CD31 in GHS were higher than those in Matrigel, Control, and low concentration G-dECM groups, while at a concentration of 6 mg/mL G-dECM, the expression levels of CD31 and VEGFA showed a decreasing trend compared to the 4 mg/mL concentration, with statistical differences observed ( Figure 4 f and g).

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The results showed that under conditions of Matrigel and low concentration G-dECM (2 mg/mL) encapsulation, an increasing trend in the mRNA and protein expression levels of VEGFA and CD31 was observed, but no statistical differences were observed.

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