Exploring the role of hydrogen peroxide in the immune microenvironment of hypoxic tumors.

Phenotypically, CD206 (Mφ2), but also CD80 (Mφ1) decreased at both time points, with CD206 marginally significant at 24 h, ( Fig. 3 D), while overall macrophage population (F4/80 + ) remained stable ( Fig. 3 A).

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Phenotypically, the CD80 + /CD206 + ratio showed an increasing trend at 24 h, while this pattern was less consistent at 96 h ( Fig. 3 E), indicating that early functional shifts may not always correspond to distinct phenotypic polarization.

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