Application of a modified MSRE-qPCR method for detecting circulating cell-free DNA methylation in cervical cancer.

Serial dilution experiments demonstrated significant disparities at moderate dilutions (1:1, p = 0.0444, Figure 3I ; 1:2, p = 0.0364, Figure 3J ), highly significant disparities at intermediate dilutions (1:10, p = 0.0004, Figure 3K ; 1:20, p = 0.0007, Figure 3L ; 1:50, p = 0.0007, Figure 3M ), and significant disparities at high dilutions (1:100, p = 0.007, Figure 3N ; 1:1000, p = 0.0047, Figure 3O ), while there was no significant difference at extreme dilution (1:10000, Figure 3P ).

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