Transcriptome and targeted metabolome analysis reveals sugar metabolism regulation in maize stalks at different densities.

As planting density increased, sucrose accumulation in the third internode of the stalk base showed an increasing trend, with extremely significant differences ( Figure 1E ).

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The expression level of the SPS1 was elevated with advancing growth stages at density D2, while SPS2 exhibited the opposite expression pattern; IVR1 and IVR2 showed little change in expression levels at different densities as the growth stage progressed in M1, but expression levels were elevated as the growth stage progressed at density D2 in M2; and BAM1 and Sh2 showed a decreasing trend in expression levels as the growth stage progressed and the stalk gradually aged.

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