Discovery of two novel mutations, R52H and N868D, in the voltage-gated sodium channel of Aedes aegypti associated with pyrethroid resistance.

The allele frequencies of A532E in G0, G3, and G6 were 0.1, 0.025, and 0.025, respectively, suggesting a decreasing trend ( Table 2 ).

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The frequency of N868D (chromatogram is shown in S1A Fig ) in G0, G3, and G6 was 0.125, 0.4, and 0.325, respectively, suggesting an increasing trend.

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