not quite statistically significantp = 0.076
We performed statistical analysis comparing the nuclear protrusion lengths at multiple time points, which revealed a significant decrease in nuclear protrusion length of M1 macrophages compared to M0 macrophages at 60 and 190 s of aspiration, and a slight but not quite statistically significant difference ( p = 0.076) at 140 s (Supplemental Fig. 5A–C ).