Historical Contingency Drives Freshwater Microbial Community Assembly Across Successional Time.

First, analyzing the effects of history and environment at fixed immigration levels revealed that historical source, environmental medium, and their interaction all had highly significant effects on community composition variation across all three immigration treatments (control, higher, highest) and at both time points ( p = 0.001, Table 1 ).

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By day 60, ASV richness exhibited a decreasing trend with immigration in both homogenous treatments, reaching significance only in Long‐Long ( p = 0.002) but not in Fen‐Fen ( p = 0.151).

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