Impact of noradrenaline, adrenergic receptors, and radical formation on cardiac mitochondria in resuscitated swine.

Immunohistochemistry was chosen, because (1) it is well established in the literature that densitometric analysis of colorimetric immunohistochemical staining is as acceptable a method as Western blotting for protein measurement [ 28 ], (2) we had previously established the immunohistochemistry protocols for porcine cardiac tissue specimens [ 13 ], (3) we had obtained highly significant correlations between the densitometric values and those obtained by Western blotting [ 29 ], and (4) in contrast to Western blotting, the immunohistochemistry evaluation of the tissue allows identification of the physical topography and protein expression in different cell types within the tissue specimen.

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