highly significantp ≤ 0.001
GraphPad Prism 9.5 software was used for plotting, and statistical significance was expressed as * p ≤ 0.05, indicating significance; ** p ≤ 0.01, highly significant; *** p ≤ 0.001, extremely significant; **** p ≤ 0.0001; ns, there is no significant difference. 5.