Multifactorial Analysis Identifies Conditions for Robust HCoV-OC43 Replication in Primary Human Bronchial Epithelial Cells Cultured at Air-Liquid Interface.

HCoV-OC43 gRNA from cell lysates and apical wash samples was evaluated within our multivariable regression model, which indicated a slight trend of higher endpoint gRNA when using passage 1 over passage 3 cells ( Figure 7 ); however, this effect was not significant.

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