Even though relied on for over a century and despite the ubiquitous nature of H&E staining, it is well recognized that this staining can result in fairly significant variations in color, due in part to the natural derivation of the stains as well as inconsistencies in staining protocol (incubation time, temperature, humidity, batch variation, etc.) that sometimes can impede pathological evaluation, although pathologists are trained to ‘read through’ these variations [ 56 – 58 ].
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AI and the digital pathology revolution: clinical applications in cancer diagnosis and assessment.
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