Screening disease feature genes and analyzing correlations with immune cell infiltration in knee osteoarthritis chondrocytes based on multiple machine learning algorithms.

Violin plots ( Fig 8A and 8B ) revealed highly significant differences in PFKFB3 and DDIT3 expression between the control and OA groups (*** P < 0.001): both genes were highly expressed in controls and significantly downregulated in OA—consistent with prior differential analysis and RF results.

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