The alanine will then react with NAD + and become converted to pyruvate and NADH by the action of alanine dehydrogenase. First, using alanine standards, we confirmed whether the initial velocity of the ADH-catalyzed reaction exhibited a positive trend with the increasing alanine concentration ( Figure 1B ).
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Development of A Spectrometric Enzyme-Coupled Assay for 11-Aminoundecanoic Acid Transaminase and Its Detection in <i>Pseudomonas</i> Strain JG-B Cell Lysate.
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