Evolution of the TCP gene family and pan-genome analysis in tea (Camellia sinensis).

To further avoid approximation error, we additionally conducted a permutation test (randomly shuffling the Core/Dispensable labels 20,000 times and recalculating χ2), which remained highly significant ( p ≈ 5.0 × 10−5) (Fig. 4 C, Table S14), supporting that “the distribution of core/dispensable genes changes significantly across subfamilies”.

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2.927) was marginally significant ( P ≈ 0.052) (Table S20), whereas Mature leaf/Old leaf/Root showed the same trend but did not reach significance ( P > 0.05) (Table S20), indicating a tendency toward higher TCP expression in cultivated materials in actively growing tissues within the compiled dataset.

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