Copper-Collagen Interactions Regulate the Mechanical and Invasive Properties of Tumor Spheroids.

Notably, the 0.025 mg mL −1 treatment condition showed highly significant differences compared to the control, while the 0.01 mg mL −1 condition showed a milder but still significant effect. 3.4 Invasive Behavior in Pre‐Formed Spheroids Exposed to Copper To further investigate copper's impact on established tumor structures, we conducted a complementary assay where copper was administered on day 6, after the spheroid formation (Figure 4a ).

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