highly significantp < 0.0001
By day 30, the DG exhibited characteristic features of granule cell dispersion (GCD), with loosening of the granule cell layer, blurring of the boundary between the granule cell layer and molecular layer, and expansion of granule cell distribution ( Figure 1 C), in line with previous observations in experimental and human TLE [ 46 , 47 ].Quantitative evaluation of NeuN immunofluorescence demonstrated a highly significant, progressive increase across the evaluated longitudinal time points from day 1 to day 30 post-modeling (One-way ANOVA, F (4, 10) = 2495.0, p < 0.0001).