highly significantp < 0.0001
The above results were subsequently analyzed using Design-Expert software, yielding the following second-order polynomial regression equation: Y = −28.06537 + 1.35037A + 1.55369B + 0.066144C − 0.007187AB + 0.00075AC + 0.000141BC − 0.169750A 2 − 0.028281B 2 − 0.000218C 2 ANOVA-based statistical validation demonstrated that the model was highly significant (F = 210.21, p < 0.0001) and exhibited strong predictive capability, with an adjusted R 2 of 0.9916, indicating good model fit.