Real Time Pulse Chase (RTPC) In-Cell NMR Spectroscopy Reveals Critical Metabolites in Subminute Metabolism of Undifferentiated and Differentiated Human Neuronal Cells.

There was a significant difference in the characteristic times resolved for glutamate between undifferentiated and differentiated cells, t ch = 33.0 vs 24.0 min, indicating faster production in differentiated cells and a highly significant difference in the binding constant, 0.010 min –1 vs 0.030 min –1 , between undifferentiated versus differentiated cells indicating more rapid clearance of glutamate from undifferentiated cells and a more extensive bound population in differentiated cells than observed for lactate or alanine ( Table , Figure ).

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