highly significantP < 0.0001
As shown in Figure 3 , rEg.P29 alone did not induce a significant increase in specific IgG at 20 weeks post-immunization, whereas the formulation with Quil A adjuvant elicited a highly significant increase in rEg.P29-specific IgG ( P < 0.0001), confirming that Quil A is essential for inducing a robust IgG-dominant humoral immune response in sheep.