highly significantp < 0.0001
The ANOVA results reveal that the treatment temperature ( B ) and treatment duration ( C ) are the most significant factors, with F -values of 306.36 and 99.49, and p -values of <0.0001 and 0.0002, respectively. The quadratic terms ( A 2 , B 2 , and C 2 ) are highly significant ( p < 0.0001), indicating a clear nonlinear relationship.