a decreasing trendP < 0.05
A total of 10 ARGs types exhibited a decreasing trend, among which 6 ARGs types were significantly decreased, including beta-lactam ( P < 0.05, R 2 = 0.61), MLS ( P < 0.05, R 2 = 0.53), quinolone ( P < 0.01, R 2 = 0.84) and tetracycline ( P < 0.05, R 2 = 0.64) (Fig. 5a ).