Brieflow: an integrated computational pipeline for high-throughput analysis of optical pooled screening data.

In the reanalysis, TVN batch correction was validated by analysis-of-variance (ANOVA) F -test for batch-associated variation, with p -values increasing from highly significant levels (10 −3 for interphase) to non-significant levels (0.52), confirming successful removal of technical variation (Fig. 3d ).

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