Tubule and microbranch variations in human dentine: A quantitative 3D study with serial block-face scanning electron microscopy.

For tubule density, tubule diameter, and microbranch density, a trend was determined by performing a simple linear regression to determine the coefficient of the regression line; a positive coefficient indicates an increasing trend, and vice versa.

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There is a decreasing trend from a maximum density of 45 × 10 3 tubules/mm 2 at the pulp cavity, to 3.5 × 10 3 tubules/mm 2 at the DEJ, indicating that tubule density decreases further from the pulp cavity.

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