highly significantp < 0.001
Two-way ANOVA revealed a highly significant main effect of stimulation frequency on the AP peak for both glutamatergic ( F (2, 42) = 30.40, p < 0.001, η p 2 = 0.59 ) and GABAergic neurons ( F (2, 42) = 49.25, p < 0.001, η p 2 = 0.70 ), demonstrating a frequency-dependent increase.