highly significantp < 0.0001
Two-way ANOVA revealed a highly significant time x virus interaction (F_{3,24} = 14.2, p < 0.0001), confirming that the observed cytoskeletal remodeling is US3-dependent rather than a consequence of generalized viral replication ( n = 3 biological replicates per condition; full ANOVA output in Supplementary Data S1 ).