highly significantp < 0.0001
Incubation with Wnt3a conditioned medium (CM) resulted in a small but statistically significant (p = 0.017) increase in Wnt pathway throughput, measured by luminescence following transient transfection of the Super Topflash reporter construct, in NCM460 cells and a robust and highly significant increase (p < 0.0001) in RKO cells (Figure 2A ).