Demonstration of a novel technique to quantitatively assess inflammatory mediators and cells in rat knee joints.

Results 3.1 Anaesthetic effects Samples from naïve animals (n = 5) which received no treatment during 7 hours of urethane anaesthesia, showed a slight trend for increased levels of cytokines, but the increases were not statistically significant for IL1α, IL1β, IL2, IL4, IL6, IL10, GM-CSF, IFNγ, or TNFα compared with samples taken from rats immediately after administration of anaesthetic (n = 10; P > 0.05, Mann Whitney) see Table 1 .

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