The origins of novel protein interactions during animal opsin evolution.

While we cannot conclusively resolve the G protein interaction in the ancestor of rhabdomeric+G o opsins or the ancestor of ciliary+rhabdomeric+G o clades, our reconstructed ancestral G protein interaction states for ciliary, rhabdomeric and G o are highly significant (P>0.95 for each) and are congruent with previous empirical biochemical studies [17] , [19] , [30] ( figure 6 ).

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