A Ca2+ influx associated with exocytosis is specifically abolished in a Paramecium exocytotic mutant.

Note that AED provokes a dramatic increase of initial rate of 45Cauptake only at 26C and not at 350C, al- though this is possible at that temperature in the absence of the ndl2 mutation (a). could be different at the nonpermissive temperature, the lack of Ca2+ uptake at 35C in the mutant is highly significant since control wild type or pwA cells grown at that tempera- ture do take up Ca2+upon AED stimulation at the same rate as at 26C or even slightly faster.

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