When CD25 − CD4 + T cells, which had been differentiated with IL-10 and IFN-α, were added at a 2:1 or 1:1 ratio (polarized:naive), a consistent and highly significant suppression was observed.
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Human CD25+CD4+ T suppressor cell clones produce transforming growth factor beta, but not interleukin 10, and are distinct from type 1 T regulatory cells.
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