Annexin A4 reduces water and proton permeability of model membranes but does not alter aquaporin 2-mediated water transport in isolated endosomes.

However, when Ca 2+ and Anx4 were added simultaneously to induce protein–membrane interactions, there was a highly significant (P < 0.001) 29% drop in the membrane water permeability from 5.83 × 10 −3 cm/s to 4.14 × 10 −3 cm/s.

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