Measurements of membrane potential, transmembrane 45Ca fluxes, cytoplasmic free Ca2+ concentration and insulin release by transplantable rat insulinoma cells maintained in tissue culture.

Increasing extracellular Ca2 + stepwise from 0 mM to 0.26 mM, and through to 20.4 mM resulted in successive increases in [Ca2+]i (each step at least P <0.05, except between 5.1 mM and 10.2mM which did not achieve significance).

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