Quenched flow analysis of exocytosis in Paramecium cells: time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells.

While the differ- ence in trichocyst content is highly significant (p < 0.0001), the amount of membranes fused is not different (even at p > 0.1; determined by U-test). dure (a serious risk, as we had learned in pilot experiments with commercial equipment) might have hampered its more general use.

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