Quantitation of the 35S-HSPG immunoprecipitated from the basal medium during the first 30- and 60-min periods showed that there was a highly significant decrease (>60 decrease) in the amount of 35S-HSPG secreted during both of these periods following overexpression of ai-3 (Table 1, 1119 Treatment "S-HSPG/30 min "S-HSPG/60 min LLC-PK,)a;.3) cells -Cd 399 f 68 1,229 t 86 +Cd 51 f 35* 479 t 88* +Cd+PTX 729 t 64* 1,684 t 117t -Cd+PTX 701 f 117 1,547 f 17411 LLC-PK.;_2) cells -Cd 277 f 28 919 t 29 +Cd 278 f 241 923 t 351 LLC-PK, cells -Cd 318 t 36 1,045 t 61 +Cd 330 f 51, 1,071 t 961 Figure 8.
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A heterotrimeric G protein, G alpha i-3, on Golgi membranes regulates the secretion of a heparan sulfate proteoglycan in LLC-PK1 epithelial cells.
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