Prognostic impact of urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1) in cytosols and pellet extracts derived from 892 breast cancer patients.

The mean and median levels of uPA as determined by both ELISAs were similar. However, the mean and median PAI-1 levels assessed by the two ELISAs differed by a factor of 10, the in-house ELISA measuring values ten times lower than the ELISA with commercial reagents. The overall association between the two ELISA methods is demonstrated by the highly significant correlations between the cytosolic levels of uPA (rs = 0.85, P < 0.0001) and of PAI-1 (rs = 0.90, P < 0.0001) (Figure 1A and B).

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