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Altered patterns of gene expression underlying the enhanced immunogenicity of radiation-attenuated schistosomes.

PLoS Negl Trop Dis · 2008 · PMC2375114 · PMID 18493602

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Validation of expression patterns Three ESTs deemed to be differentially expressed, using the LIMMA package of Bioconductor, plus one on the threshold of significance were chosen for validation of array predictions by real time PCR analyses.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.