highly significantP < 0.0001
Statistical analyses Microsatellite diversity, Hardy-Weinberg and genotypic linkage equilibrium GenePop 3.4 [ 14 ] was initially employed to screen for deviations from Hardy-Weinberg (H-W) equilibrium and two loci (BFRO4 and ONE2) were excluded due to highly significant heterozygote deficiency (P < 0.0001) in several populations, likely indicating the presence of null alleles.