RT-PCR and quantitative Real-time PCR validation In order to confirm the DEGs detected by exon array, 14 DEGs exhibiting highly significant differences in expression or with important functions (for example, splicing factors) were validated by RT-PCR in Figure S1 (See Additional file 4 ).
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Transcription and splicing regulation in human umbilical vein endothelial cells under hypoxic stress conditions by exon array.
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