In males, one of the lines (E) showed a small, marginally significant, increase in median lifespan ( Fig. 4 B), replicated with line F ( Fig. 4 D), but no effect on maximum lifespan ( Supplementary Table 1 ).
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Expression of human uncoupling protein-3 in Drosophila insulin-producing cells increases insulin-like peptide (DILP) levels and shortens lifespan.
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