Methods RNA aptamer and systematic evolution of ligands by exponential enrichment (SELEX): The SELEX selection procedure was utilized to isolate candidate OPN aptamers, as described previously.[ 7 , 8 ] After each round of SELEX, we performed a binding affinity assay to measure the aptamer pool's Kd value to ensure that the Kd values exhibited a decreasing trend.
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Identification of osteopontin-dependent signaling pathways in a mouse model of human breast cancer.
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