New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Forster resonance energy transfer probes in 5'-nuclease assays.

Nucleic Acids Res · 2010 · PMC2836566 · PMID 19969535

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may not be significantno p-value reported

However, these positive changes may not be significant for routine use in Snake, especially taking into account the associated increase in the overall assay time (up to ∼30–55%).

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