Correlated with this, a dramatic and highly significant change in the width of the unfolded state of the trapped intermediate is observed in mildly denaturing conditions ( Fig. 7 a and b, center panels, triangles), which is not observed for the folded state observed in the same experiment, obviating instrumental or processing artifacts as the cause. 34 For both Im7 I eqm H1H4 and Im7 I eqm GlyH1H3 at denaturant concentrations between 1 and 3 M, the distribution width increases as the inter-dye separation decreases.
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