Effect of reference genes on relative expression of target miRNAs Of the four target miRNAs evaluated after normalisation to different references, the choice of reference gene did not influence the relative quantity of miR-31 ( p < 0.001) between tumour and normal tissues suggesting a highly significant differential expression of miR-31 in CRC (Figure 4b ).
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MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer.
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