Differences in PLUNC mRNA levels were confirmed by quantitative (q) RT-PCR to be significantly decreased in KO lungs by 95%, whereas two other microarray targets close to significance, TFF2 and Reg3γ, which were decreased by three- and fivefold in KO lungs, respectively, did not display differences by qRT-PCR ( Fig. 5 A ).
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Epithelial reticulon 4B (Nogo-B) is an endogenous regulator of Th2-driven lung inflammation.
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