Role of carbonic anhydrase IV in the bicarbonate-mediated activation of murine and human sperm.

The addition of 50 nM EZA significantly decreases enzymatic activity from 7.01±0.46 U/ml to 4.53±0.87 U/ml (35.38% reduction), 100 nM EZA leads to a highly significant decrease from 6.29±0.57 U/ml to 3.15±0.46 U/ml (49.92% reduction) and 5 μM EZA reduces enzymatic activity from 7.18±0.20 U/ml to 2.72±0.12 U/ml (62.12% reduction).

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