Comparison of sequence length, quality and GC content of the set of sequences with and without nr BLAST hits showed highly significant differences (Table 3 ): annotated sequences were longer and of higher quality than non-annotated sequences, and GC content of the two sets was on average about 8 percent points higher in annotated sequences.
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Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered European eel.
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