As an additional evaluation of the quality of our assembly and sequencing, we identified potential chimeric sequences that are made-up of two unique sequences that have been concatenated such that the sequence had two highly significant (< 1e -20 ) NCBI-NR hits to different genes, which aligned to different ends of the contig or singleton.
← all excerpts
A garter snake transcriptome: pyrosequencing, de novo assembly, and sex-specific differences.
1
—
—