SigCleave analysis of DIA1R (data not shown) provided a highly significant score for cleavage between amino acids 31 and 32 (CSFS-LPAS, using single-letter amino-acid code, where the hyphen indicates the site of cleavage) of 10.23 (where values of greater than 3.5 are considered significant), suggesting this may represent the true site of SP cleavage for human DIA1R.
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The sentences
The NN algorithm ( Figure S1B ) only predicted a single DIA1R cleavage site (between amino acids 31 and 32) with a Y-score close to significance (a Y-score of 0.44, where a Y-max value of 0.5 is considered significant).