Ambiguous nucleotide calls from population-based sequencing of HIV-1 are a marker for viral diversity and the age of infection.

Clin Infect Dis · 2011 · PMC3060900 · PMID 21220770

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closest p · 0.0× alpha

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highly significantP < .001actually significant

Moreover, for the ZPHI set, we observed a highly significant correlation (Spearman ρ = 36; P < .001) between the fraction of ambiguous nucleotides in the pol gene and the diversity of clonal env sequences [ 14 ], which confirms that the fraction of ambiguous nucleotides is a good marker for viral diversity.

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a nonsignificant trendno p-value reported

There was a nonsignificant trend for higher viral diversity among carriers of homozygous HLA-B alleles of .243 (95% CI -.054 to .551).

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